These guidelines apply only to employers and individuals who come under the regulatory authority of the U.S. Department of Transportation (DOT) and those individuals who conduct urine specimen collections under DOT regulations. The term “employee” is used throughout this document and has the same meaning as “donor” as used on the Federal Drug Testing Custody and Control Form (CCF).

These guidelines are a complete revision of the DOT Urine Specimen Collection Procedures Guidelines, 49 CFR Part 40, for Transportation Workplace Drug Testing Programs.  Download Guideline

Note: All items that are bolded, underlined, and italicized in this revision reflect the changes to the collection procedures and requirements from the Interim Final Rule, published on September 27, 2010, effective October 1, 2010.